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( A–E ) The mRNA levels of <t>SREBP-1c,</t> ACC1, FASN, PPARα, and CPT1A in pancreatic tissues measured by RT-qPCR (n=3, * P <0.05, ** P <0.01, *** P <0.001, ns indicates no statistical significance).
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( A–E ) The mRNA levels of SREBP-1c, ACC1, FASN, PPARα, and CPT1A in pancreatic tissues measured by RT-qPCR (n=3, * P <0.05, ** P <0.01, *** P <0.001, ns indicates no statistical significance).

Journal: Journal of Inflammation Research

Article Title: Qingyi Decoction Alleviates Alcoholic Pancreatitis by Improving Glycerolipid Homeostasis via the AMPK/SREBP-1c/PPARα Pathway

doi: 10.2147/JIR.S537259

Figure Lengend Snippet: ( A–E ) The mRNA levels of SREBP-1c, ACC1, FASN, PPARα, and CPT1A in pancreatic tissues measured by RT-qPCR (n=3, * P <0.05, ** P <0.01, *** P <0.001, ns indicates no statistical significance).

Article Snippet: Subsequently, membranes were blocked with a 5% skim milk solution for 1.5 h. Antibodies AMPKα (Rabbit, Proteintech, 1:1000), p-AMPKα (Rabbit, CST, 1:1000), SREBP-1c (Rabbit, Affinity, 1:1000), ACC1 (Rabbit, ABclonal, 1:1000), FASN (Rabbit, Proteintech, 1:5000), PPARα (Rabbit, Proteintech, 1:1000) and CPT1A (Mouse, Affinity, 1:5000) were added overnight at 4°C, and horseradish peroxidase (HRP) conjugated secondary antibodies (ABclonal, 1:10000) was incubated for 1 h at room temperature.

Techniques: Quantitative RT-PCR

( A–F ) The protein levels of AMPKα, p-AMPKα, SREBP-1c, ACC1, FASN, PPARα, and CPT1A in pancreatic tissues detected by Western blot (n=3, * P <0.05, ** P <0.01, *** P <0.001, ns indicates no statistical significance).

Journal: Journal of Inflammation Research

Article Title: Qingyi Decoction Alleviates Alcoholic Pancreatitis by Improving Glycerolipid Homeostasis via the AMPK/SREBP-1c/PPARα Pathway

doi: 10.2147/JIR.S537259

Figure Lengend Snippet: ( A–F ) The protein levels of AMPKα, p-AMPKα, SREBP-1c, ACC1, FASN, PPARα, and CPT1A in pancreatic tissues detected by Western blot (n=3, * P <0.05, ** P <0.01, *** P <0.001, ns indicates no statistical significance).

Article Snippet: Subsequently, membranes were blocked with a 5% skim milk solution for 1.5 h. Antibodies AMPKα (Rabbit, Proteintech, 1:1000), p-AMPKα (Rabbit, CST, 1:1000), SREBP-1c (Rabbit, Affinity, 1:1000), ACC1 (Rabbit, ABclonal, 1:1000), FASN (Rabbit, Proteintech, 1:5000), PPARα (Rabbit, Proteintech, 1:1000) and CPT1A (Mouse, Affinity, 1:5000) were added overnight at 4°C, and horseradish peroxidase (HRP) conjugated secondary antibodies (ABclonal, 1:10000) was incubated for 1 h at room temperature.

Techniques: Western Blot